![]() Lastly, data processing and putative metabolite identification with the use of the open-source software MetaboliteDetector is shown. ![]() ![]() After solvent-based extraction of volatile metabolites contained therein, the necessary steps for analyzing the samples via liquid-injection-GC-MS are presented. The dissection procedure is performed under cooled conditions and without the use of any dissection buffer solution to minimize the changes in the chemical composition of the MGR contents. We show the dissection of worker ants of this species for the isolation of the gastral portion of the wax-like MGR contents as well as listing the necessary steps required for solvent-extraction of the therein contained volatile compounds with subsequent gas chromatography-mass spectrometry (GC-MS) analysis and putative identification of metabolites contained in the extract. In territorial combat, they use the viscous contents of their enlarged mandibular gland reservoirs (MGRs) to kill rival arthropods in characteristic suicidal 'explosions' by voluntary rupture of the gastral integument (autothysis). Ants belonging to the minor worker caste possess distinctive hypertrophied mandibular glands (MGs). The aim of this manuscript is to present a protocol describing the metabolomic analysis of Bornean 'exploding ants' belonging to the Colobopsis cylindrica (COCY) group. ![]()
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